Serum Samples:
- Following standard precautions for collecting biological specimen, collect 5 to 7 ml whole blood in a properly labelled (with patient or animal ID & date) blood collection tube.
- Separate serum following standard serum separation methods.
- Transfer 1.0 – 2.0 ml of serum to a 2 ml cryovial labelled with patient or animal ID and date of sample collection.
- Either ship immediately following shipping instructions provided in this document or store at appropriate storage temperatures.
- Serum samples can be stored refrigerated (2.0 – 6.0° C) up to one week.
- Serum samples can be stored frozen (≤ -20.0 ° C) for extended periods of time.
Swab Samples:
- Following standard precautions for collecting biological specimen, using a sterile swab, swab each collection site (if applicable) with a new swab and place in an accurately labeled (patient or primate ID, date and swab site) viral transport sample tube.
- Break the shafts if longer than the tube and seal the tube properly to prevent leaks and possible exposure to B virus.
- Either ship immediately following shipping instructions provided in this document or store at appropriate storage temperatures.
- Swab samples can be stored refrigerated (2.0 – 6.0° C) up to one week.
- Swab samples can be stored frozen (≤ -70.0 ° C) for extended periods of time.
Note: Only appropriately trained individuals should collect the specimens.
| Day Zero or Base Line Samples | Human Baseline Serum (2.0 mL – 3.0 mL) collected at the Time of Injury or as soon as possible after injury |
| Primate Baseline Serum (1.0 mL – 3.0 mL) collected at the Time of Injury or as soon as possible after injury | |
| Human Day Zero swab sample from the buccal cavity and ship the sample in Viral Transport Media tube. | |
| Primate Day Zero swab samples of the buccal cavity, eye or genitalia. Separate swab samples should be collected for each site and sent in separate Viral Transport Media tubes. | |
| Post Seroconversion/ Follow-up Samples (2-3 Weeks Post Exposure) | Human Follow-up Serum (2.0 mL – 3.0 mL) collected 2-3 weeks after Injury |
| Primate Follow-up Serum (1.0 mL – 3.0 mL) collected 2-3 weeks after Injury | |
| Human Follow up swab samples of the wound/lesion (if applicable) or buccal cavity collected 2-3 weeks after Injury and sent in separate Viral Transport Media tubes. | |
| Primate Follow up swab samples of the buccal cavity, eye or genitalia, collected for each site 2-3 weeks after incident and sent in separate media tubes. | |
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ATTENTION:
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| Test Description | Order Code |
Pricing |
Methodology | Sample Type |
| Human – B Virus Routine Surveillance | HS01 | $175.00 | ELISA | Serum |
| HS02 | $200.00 | PCR | Buccal Swab/ Wound Swab/Lesion Swab/ Eye Swab | |
| Human – B Virus Exposure – Baseline | HB01 | $175.00 | ELISA | Serum |
| HB02 | $200.00 | PCR | Buccal Swab/ Wound Swab/Lesion Swab/Eye Swab | |
| Human – B Virus Exposure – Follow up | HF01 | $175.00 | ELISA | Serum |
| HF02 | $200.00 | PCR | Buccal Swab/ Wound Swab/Lesion Swab/ Eye Swab | |
| NHP – B Virus Incident – Baseline | NB01 | $115.00 | ELISA | Serum |
| NB02 | $200.00 | PCR | Buccal Swab/ Wound Swab/Lesion Swab/ Eye Swab | |
| NHP – B Virus Incident – Follow up | NF01 | $115.00 | ELISA | Serum |
| NF02 | $200.00 | PCR | Buccal Swab/ Wound Swab/Lesion Swab/ Eye Swab | |
| Additional Confirmation – Upon Request Only | BWB | $175.00 | Western Blot | Serum |
Q. Why are 2 serum samples (Day Zero and Follow-up) needed from an individual who potentially got exposed to B virus in an incident?
Paired (two) samples are required because the first sample (Day Zero) represents the serum status at the time of potential exposure/injury. Since B virus exposure is not common in humans, antibodies against B virus will be absent in most individuals at the time of incident. The second (follow-up) sample is taken after certain time required for antibody generation if exposure happened. The Day Zero sample sets the baseline, and the second sample provides the difference in antibody levels or presence of B virus DNA if exposure occurred. If no difference is observed between the two samples, most likely exposure did not happen.
Q. Can I collect the swab samples from the exposure/injury associated monkey 1-2 days or more post the incident?
Swab samples collected right after the incident provide information about the infection status of the monkey at the time of exposure/injury and inform if the monkey was shedding virus at the time of incident. If samples are collected 1-2 or more days later it may impact the accuracy of the results and result in misinformation, as it is possible for the monkey to shed the virus intermittently. It is of paramount importance to determine if viruses are being shed at the time of exposure. We encourage that the swab samples be collected as soon as possible post exposure.
Q. How to store samples collected on exposure that occurred on a weekend or a holiday, until they are shipped?
The serum samples can be stored refrigerated (2.0 – 6.0° C) up to one week and stored frozen (≤ -20.0 ° C) for extended periods of time. Similarly, the Swab samples can be stored refrigerated (2.0 – 6.0° C) up to one week and stored frozen (≤ -70.0 ° C) for extended periods of time.
Q. Does immediate placement of exposed individual on prophylaxis treatment affect the recommendations for sample collection?
A. Yes. In this case a second follow-up serum sample collection is recommended, 14 to 21 days after the start of medication. Thus, in total 3 serum samples are required from individuals who are on treatment. 1– baseline sample (Day Zero); 2– first follow-up (2-3 Weeks Post Exposure); 3- second follow-up sample (2-3 Weeks Post start of treatment).